Comparative analysis of the early transcriptome of Brucella abortus - Infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis

作者：Rossetti C A; Galindo C L; Everts R E; Lewin H A; Garner H R; Adams L G^*

来源：Research in Veterinary Science, 2011, 91(1): 40-51.

DOI：10.1016/j.rvsc.2010.09.002

• Brucella
• Macrophages
• Cattle
• Resistance
• Microarray

Brucellosis is a worldwide zoonotic infectious disease that has a significant economic impact on animal production and human public health. We characterized the gene expression profile of B. abortus-infected monocyte-derived macrophages (MDMs) from naive cattle naturally resistant (R) or susceptible (S) to brucellosis using a cDNA microarray technology. Our data indicate that (1) B. abortus induced a slightly increased genome activation in R MDMs and a down-regulated transcriptome in S MDMs, during the onset of infection, (2) R MDMs had the ability to mount a type 1 immune response against B. abortus infection which was impaired in S cells, and (3) the host cell activity was not altered after 12 h post-B. abortus infection in R MDMs while the cell cycle was largely arrested in infected S MDMs at 12 h p.i. These results contribute to an improved understanding of how host responses may be manipulated to prevent infection by brucellae.

• 出版日期2011-8