Unstructured model for free and immobilized cell culture without pH control of Bifidobacterium animalis subsp lactis Bb 12-Inhibitory effect of the undissociated organic acids

作者：Jalili Hasan; Balannec Beatrice; Razavi Hadi; Amrane Abdeltif^*

来源：Biochemical Engineering Journal, 2011, 58-59: 184-188.

DOI：10.1016/j.bej.2011.09.007

• Bifidobacterium
• Batch cultures
• Kinetic models
• Organic acids
• Product inhibition

A model was developed to describe growth and organic acids production of Bifidobacterium animalis growing without pH control in free and immobilized cell culture. The Verlhust model was considered for growth, and to account for the inhibition observed at acidic pH, the Luedeking-Piret production model was modified by introducing an additional term involving the undissociated form of the organic acids, acetic and lactic acids, the main inhibitory species. To describe the relationship between pH and both the dissociated and the undissociated forms of organic acids, the Henderson-Hasselbach equation was considered. The model was found to satisfactory describe experimental growth and production data recorded during free and immobilized cell cultures. The part of each acid produced can be deduced from the calculated production data, since a constant lactic to acetic acid mass ratio was found, 1.29 and 1.66 during free and immobilized cell cultures. Owing to the acidic pH values recorded, 4.43 at lowest, higher amounts of undissociated acetic acid were produced, leading to a higher inhibitory effect of this acid if compared to lactic acid.

• 出版日期2011-12-15