A novel approach to investigate vascular wall in 3D: Combined Raman spectroscopy and atomic force microscopy for aorta en face imaging

作者：Pilarczyk Marta; Rygula Anna; Kaczor Agnieszka^*; Mateuszuk Lukasz; Maslak Edyta; Chlopicki Stefan; Baranska Malgorzata

来源：Vibrational Spectroscopy, 2014, 75: 39-44.

DOI：10.1016/j.vibspec.2014.09.004

• Vessel wall condition
• Murine aorta
• Aorta en face
• Raman spectroscopy
• Atomic force microscopy
• AFM

A novel approach based on the combination of Raman confocal 3D imaging with atomic force microscopy (AFM) for analysis of the murine vessel wall enlace is described. The approach is based on subsequent Raman and AFM imaging of the same areas of the sample. This methodology allows for direct correlation of the chemical structure (Raman data) with morphology of the surface (AFM). The sub-cellular structures of the tissue e.g., cell nuclei, heme, or lipid-rich species are visualized and localized by the application of Raman imaging, while AFM complements these data with high-resolution information about the surface topography and size of lipid-rich structures. Overall, the applied approach enables detailed characterization of the inner layer of the vessel wall.

• 出版日期2014-11