A novel LC-MS/MS method for determination of tissue distribution and excretion of timosaponin B-II in rat biological matrices

作者：Xu, Zhongyuan; Gao, Shouhong; Cai, Fei; Zhang, Feng; Jiang, Wei; Liu, Xiaowei; Chen, Wansheng^*

来源：Biomedical Chromatography, 2014, 28(7): 1011-1016.

DOI：10.1002/bmc.3109

• timosaponin B-II
• tissue distribution
• excretion
• rats
• LC-MS/MS

Timosaponin B-II (TB-II) is a natural bioactive steroid glycoside extracted from the Chinese medicinal herb Anemarrhena asphodeloides Bge. (Fam. Liliaceae). It has been demonstrated to have a good anti-inflammatory effect and a low bioavailability (1.1%). Clinical research has focused on developing it into a completely new medicine. In this study, a rapid and sensitive analytical method based on LC-MS/MS has been developed for the determination of TB-II in rat biological matrices (tissues, bile, urine and feces samples). The analytes and internal standard were isolated from 100 mu L samples by solid-phase extraction and then separated using a DIKMA Inertsil ODS-3 column (5 mu m, 2.1 x 150 mm) with an isocratic mobile phase consisting of acetonitrile-0.05% formic acid (35:65) at a flow rate of 0.25mL/min. Calibration curves (1/chi(2)-weighted) offered satisfactory linearity (r(2)>= 0.990) within the test range. The accuracy, precision, recoveries and matrix effects were satisfactory in all the biological matrices examined. The assay was successfully applied to a tissue distribution and excretion study in rats. The preclinical data are useful for the design of clinical trials of TB-II.

• 出版日期2014-7
• 单位中国人民解放军第二军医大学; 天津市中医药研究院附属医院; 南方医科大学