Adjuvant effect of polysaccharide from fruits of Physalis alkekengi L. in DNA vaccine against systemic candidiasis

作者：Yang, Huimin; Han, Shuying; Zhao, Danyang; Wang, Guiyun^*

来源：Carbohydrate Polymers, 2014, 109: 77-84.

DOI：10.1016/j.carbpol.2014.03.054

• Physalis alkekengi L. polysaccharide
• Adjuvant
• Candida albicans
• DNA vaccine

Adjuvant effect mediated by polysaccharide (PPSB) isolated from the fruits of Physalis alkekengi L in DNA vaccine was evaluated in mice. Recombinant plasmid containing epitope C (LKVIRK) from heat shock protein 90 (HSP90) of Candida albicans (C. albican) was used as DNA vaccine (pD-HSP90C). The results indicated that PPSB significantly enhanced specific antibody titers IgG, IgGl, IgG2b, and concentration of IL-2 and IL-4 in sera of mice immunized with pD-HSP90C (p<0.05). More importantly, it was found that the mice immunized with pD-HSP90C/PPSB not only had fewer CFU (colony forming unites) in the kidneys than mice immunized with pD-HSP90C, but also a statistically significant higher survival rate over PBS-injected group (p<0.05) when the immunized mice were challenged with living C albican cells. However, no statistically significant difference in survival rate was observed between pD-HSP90C-immunized group and PBS-injected group. Therefore, PPSB can be considered as a promising adjuvant eliciting both Th1 and Th2 responses to enhance the efficacy of DNA vaccines.

• 出版日期2014-8-30
• 单位东北师范大学