科研之友
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摘要
A rapid, simple and sensitive HPLC method for the quantification of levobupivacaine in plasma was developed and validated. The analysis involved a simple liquid-liquid extraction. Plasma was extracted with hexane and the organic extract was then evaporated and the residue was reconstituted in mobile phase. The reconstituted solution was injected into an HPLC system and was subjected to reverse-phase HPLC on a 5 mu m C(18) column at a flow-rate of 1 ml/min. The mobile phase consisted of potassium dihydrogen phosphate (0.01 mol/L) and acetonitrile (85:15 pH = 4.0). Standard curves were linear over the concentration range of 0.0125 to 2 mg/L. The mean predicted concentrations of the quality control (QC) samples deviated by less than 2% from the corresponding nominal values; the intra-assay and inter-assay precision of the assay were within 6% relative standard deviation. The extraction recovery of levobupivacaine was more than 85%. The validated assay was applied to a pharmacokinetic study of levobupivacaine in plasma in Chinese patients with normal renal function or renal disease.
- 出版日期2011-11
- 单位同济大学; 同济大学附属上海市肺科医院
