A duplex PCR for the rapid and simultaneous detection of Brucella spp. in human blood samples

作者：Mirnejad Reza; Mohamadi Mozafar; Piranfar Vahbeh^*; Mortazavi Seied Mojtaba; Kachuei Reza

来源：Asian Pacific Journal of Tropical Medicine, 2013, 6(6): 453-456.

DOI：10.1016/S1995-7645(13)60073-5

• Brucella abortus
• Brucella melitensis
• Brucella ovis
• Duplex PCR

Objective: To design a duplex PCR for rapid and simultaneous detection of Brucella species. in human blood samples. Methods: Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis. Following DNA extraction, PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals. Results: Of the 52 peripheral bloods samples tested, 25 sample (48%) showed positive reactions in PCR. Twelve samples were positive for Brucella abortus (B. abortus) (23%), 13 for Brucella melitensis (B. melitensis) (25%) and 0 for Brucella ovis B. ovis) (0%). Conclusions: This work de=monstrates that in case where specific primers were utilized, duplex PCR has proved to be a simple, fast, and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples.

• 出版日期2013-6