Antioxidant and Electron Donating Function of Hypothalamic Polypeptides: Galarmin and Gx-NH2

作者：Tavadyan L A^*; Galoian K A; Harutunyan L A; Tonikyan H G; Galoyan A A

来源：Neurochemical Research, 2010, 35(6): 947-952.

DOI：10.1007/s11064-010-0173-8

• Proline-rich peptide
• Electron donator
• Antioxidant
• Antiradical activity
• Chelator
• DPV

Chemical mechanisms of antioxidant and electron donating function of the hypothalamic proline-rich polypeptides have been clarified on the molecular level. The antioxidant-chelating property of Galarmin and Gx-NH2 was established by their capability to inhibit copper(II) dichloride catalyzed H2O2 decomposition, thus preventing formation of HOaEuro cent and HOOaEuro cent radicals. The antiradical activity of Galarmin and Gx-NH2 was determined by their ability to react with 2,2-diphenyl-1-picrylhydrazyl radical applying differential pulse voltammetry and UV-Vis spectrophotometry methods. Galarmin manifest antiradical activity towards 2,2-diphenyl-1-picrylhydrazyl radical, depending on the existence of phenolic OH group in tyrosine residue at the end of the molecule. The presence of antiradical activity and reduction properties of Galarmin are confirmed by the existence of an oxidation specific peak in voltammograms made by differential pulse voltammetry at E (a similar to) = 0.795 V vs. Ag/Ag+ aq.

• 出版日期2010-6