Association study of the TNF-alpha-1031T/C and VEGF+450G/C polymorphisms with susceptibility to endometriosis

作者：Saliminejad Kioomars; Memariani Toktam; Ardekani Ali M; Kamali Koorosh; Edalatkhah Haleh; Pahlevanzadeh Zhamak; Khorshid Hamid Reza Khorram^*

来源：Gynecological Endocrinology, 2013, 29(11): 974-977.

DOI：10.3109/09513590.2013.824956

• Endometriosis
• PCR-RFLP
• polymorphism
• TNF-alpha
• VEGF

Tumor necrosis factor-alpha (TNF-alpha), a multifunctional proinflammatory cytokine, and vascular endothelial growth factor (VEGF), a major mediator of angiogenesis and vascular permeability, have been investigated in endometriosis patients of different populations. This study was carried out to investigate whether the two polymorphisms, TNF-alpha -1031T/C and VEGF_450G/ C are associated with susceptibility to endometriosis in an Iranian population. Totally, 135 women with diagnosis of endometriosis and 173 women with no evidence of the disease were included in this study. The 1031T/C and _450G/C polymorphisms were assessed by PCR- RFLP analysis, using the two restriction enzymes BbsI and BsmFI, respectively. The frequencies of the TNF-alpha 1031TC genotype (p 0.038) and the 1031 C allele (p 0.048) were significantly lower in patients than control group. In contrast, no significant differences in the genotype and allele frequencies of the VEGF_450G/C polymorphism were found between the case and control groups. Our results suggest that the TNF- alpha - 1031T/C polymorphism was associated with susceptibility to endometriosis in Iranian population, and the - 1301C allele may have a protective role in development of endometriosis; On the contrary, we find no association between the VEGF+450G/C polymorphism and risk of endometriosis.

• 出版日期2013-11