Detection of Listeria monocytogenes in ready-to-eat food by Step One real-time polymerase chain reaction

作者：Pochop Jaroslav; Kacaniova Miroslavaka^*; Hleba Lukas; Lopasovsky L'Ubom; Bobkova Alica; Zelenakova Lucia; Stricik Michal

来源：Journal of Environmental Science and Health - Part B: Pesticides, Food Contaminants, and Agricultural Wastes , 2012, 47(3): 212-216.

DOI：10.1080/03601234.2012.634353

• Real-time PCR
• Listeria monocytogenes
• detection kit
• ready-to-eat food

The aim of this study was to follow contamination of ready-to-eat food with Listeria monocytogenes by using the Step One real time polymerase chain reaction (PCR). We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ (R) Listeria monocytogenes Detection Kit for the real-time PCR performance. In 30 samples of ready-to-eat milk and meat products without incubation we detected strains of Listeria monocytogenes in five samples (swabs). Internal positive control (IPC) was positive in all samples. Our results indicated that the real-time PCR assay developed in this study could sensitively detect Listeria monocytogenes in ready-to-eat food without incubation.

• 出版日期2012