Hsp70 and lipid peroxide levels following heat stress in Xeropicta derbentina (Krynicki 1836) (Gastropoda, Pulmonata) with regard to different colour morphs

作者：Dieterich A^*; Troschinski S; Schwarz S; Di Lellis M A; Henneberg A; Fischbach U; Ludwig M; Gaertner U; Triebskorn R; Koehler H R

来源：Cell Stress & Chaperones, 2015, 20(1): 159-168.

DOI：10.1007/s12192-014-0534-3

• FOX assay
• Heat stress
• Hsp70 level
• Lipid peroxidation
• Shell colouration

Terrestrial snails which live under dry and hot conditions need efficient mechanisms of adaptation to counteract the problems of desiccation and over-heating. A profoundly heat tolerant snail species is the Mediterranean Xeropicta derbentina, exhibiting different shell colour morphs ranging from pale white to darkly banded. Considering that dark-pigmented snails are believed to have a disadvantage due to faster heating, we investigated possible differences in the stress markers Hsp70 and lipid peroxideation between four pre-defined colour morphs which were exposed to different temperatures for eight hours. The highest Hsp70 levels were observed in response to 38-40 A degrees C. Levels decreased when this temperature was exceeded. Snails of a pre-defined colour category 3 (with a large black band at the umbilicus side of the shell) showed the most prominent Hsp70 response. Lipid peroxideation levels also showed a maximum at 38 A degrees C but displayed a second peak at rather high temperatures at which the Hsp70 level already had decreased (45-48 A degrees C). Particularly pure white snails (category 1) and the most pigmented ones (category 4) were found to have different levels of lipid peroxidation at 38 A degrees C and 45 A degrees C compared to the other morphs. A hypothesis involving a combined two-phase defence mechanism, to which both, the Hsp70 protection system and the antioxidant defence system, may contribute, is discussed.

• 出版日期2015-1