High intra-individual sequence variation in the nuclear rDNA LSU-5S intergenic spacer in the Sargassaceae (Fucales, Phaeophyceae)

作者：Draisma Stefano G A^*; Eurlings Marcel C M; Lim Phaik Eem

来源：Journal of Applied Phycology, 2012, 24(6): 1373-1379.

DOI：10.1007/s10811-012-9789-1

• Alignment-free analysis
• Intra-individual variation
• Linked 5S
• rDNA intergenic spacer
• Pseudogenic
• Sargassaceae
• Tandem repeats

Of four species of Sargassaceae, representing the genera Carpophyllum, Cystoseira, Landsburgia, and Sargassum, the intergenic spacer of the ribosomal cistron was amplified using a forward primer annealing at the 3%26apos;-end of the large subunit (LSU) of the ribosomal cistron and a reverse primer annealing at the 5S rRNA gene. The PCR products were cloned and the DNA sequences of multiple clones were determined. Almost each clone showed a unique DNA sequence. Intra-individual variation of this LSU-5S intergenic spacer was extremely high and was characterized by great length variation and a high number of short tandem repeats. Sequences were unalignable and therefore it was concluded that the LSU-5S intergenic spacer is unsuitable for phylogenetic and phylogeographic studies of sargassacean taxa.

• 出版日期2012-12