Mouse oocytes depend on BubR1 for proper chromosome segregation but not for prophase I arrest

作者：Touati Sandra A; Buffin Eulalie; Cladiere Damien; Hached Khaled; Rachez Christophe; van Deursen Jan M; Wassmann Katja^*

来源：Nature Communications, 2015, 6(1): 6946.

DOI：10.1038/ncomms7946

Mammalian female meiosis is error prone, with rates of meiotic chromosome missegregations strongly increasing towards the end of the reproductive lifespan. A strong reduction of BubR1 has been observed in oocytes of women approaching menopause and in ovaries of aged mice, which led to the hypothesis that a gradual decline of BubR1 contributes to age-related aneuploidization. Here we employ a conditional knockout approach in mouse oocytes to dissect the meiotic roles of BubR1. We show that BubR1 is required for diverse meiotic functions, including persistent spindle assembly checkpoint activity, timing of meiosis I and the establishment of robust kinetochore-microtubule attachments in a meiosis-specific manner, but not prophase I arrest. These data reveal that BubR1 plays a multifaceted role in chromosome segregation during the first meiotic division and suggest that age-related decline of BubR1 is a key determinant of the formation of aneuploid oocytes as women approach menopause.

• 出版日期2015-4