MTA1 promotes the invasion and migration of non-small cell lung cancer cells by downregulating miR-125b

作者：Li, Yiyi; Chao, Yilan; Fang, Yuan; Wang, Jian; Wang, Min; Zhang, Hong; Ying, Min; Zhu, Xiaoxia^*; Wang, Haofei

来源：Journal of Experimental & Clinical Cancer Research, 2013, 32: 33.

DOI：10.1186/1756-9966-32-33

• miR-125b
• MTA1
• Non-small cell lung cancer
• Metastasis

Background: The metastasis-associated gene 1 (MTA1) has been identified as one critical regulator of tumor metastasis. Previously, we identified miR-125b as a downregualted miRNA in non-small cell lung cancer (NSCLC) cell line upon MTA1 depletion. However, the role of miR-125b and MTA1 in the regulation of NSCLC metastasis remains unclear. @@@ Methods: Stable MTA1 knockdown NSCLC cell lines 95D and SPC-A-1 were established by transfection with MTA1 shRNA. The effects of MTA1 depletion on the expression of miR-125b and cell migration and invasion were examined by real-time PCR, wound healing and matrigel invasion assay. @@@ Results: MTA1 knockdown led to the upregulation of miR-125b level in NSCLC cells. Furthermore, MTA1 knockdown reduced while miR-125b inhibitor enhanced cell migration and invasion of NSCLC cells. Notably, miR125b inhibitor antagonized MTA1 siRNA induced inhibition of cell migration and invasion. @@@ Conclusion: MTA1 and miR-125b have antagonistic effects on the migration and invasion of NSCLC cells. The newly identified MTA1-miR-125b axis will help further elucidate the molecular mechanism of NSCLC progression and suggest that ectopic expression of miR-125b is a potentially new therapeutic regimen against NSCLC metastasis.

• 出版日期2013-5-29
• 单位南方医科大学