Antioxidant and binding properties of methanol extracts from indigo plant leaves

作者：Park Yun Jum; Shin Chang Sik; Kim Bo Eun; Cheon Gil Yong; Bae Jong Hyang; Ku Yang Gyu; Park Su Min; Heo Buk Gu^*; Kim Dae Guk; Cho Ja Yong; Gorinstein Shela

来源：CHEMICAL PAPERS, 2014, 68(10): 1421-1427.

DOI：10.2478/s11696-014-0572-4

• indigo leaves
• bioactive compounds
• antioxidant activity
• binding properties

This study was conducted to clarify the effect of ultra-pressure treatment on the extraction of bioactive compounds from indigo plant leaves (Polygonum tinctorium Lour.) and on their properties. Leaves were harvested the in mid-August, 2013, from Naju City (Korea), and treated using two methods: ultra-pressure (550 MPa, 3 min) and hot-air (70A degrees C, 24 h). Then, the leaves were ultrasonically extracted with methanol. The content of indirubin in leaves treated with ultra pressure and hot air was (535.55 +/- 26.14) mg kg(-1) and (52.63 +/- 6.45) mg kg(-1), respectively, and that of tryptanthrin was (165.55 +/- 8.74) mg kg(-1) and (153.00 +/- 7.62) mg kg(-1), respectively. Polyphenolic content in the leaves extract was (127.24 +/- 13.67) mg kg(-1) after the ultrapressure and (88.22 +/- 5.33) mg kg(-1) after the hot-air treatment. The content of flavonoids was (2298.67 +/- 83.27) mg kg(-1) after the ultra-pressure and (3224.00 +/- 21.45) mg kg(-1) after the hotair treatment. Di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) radical scavenging activities of the indigo extract obtained by ultra-pressure and hot-air treatment methods at the concentration of 1 mg mL(-1) were (80.25 +/- 0.73) % and (66.54 +/- 2.35) %, respectively, and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activities were estimated as (90.14 +/- 0.79) % and (64.45 +/- 8.97) %, respectively. The methanol leaf extract after ultra-pressure treatment exhibited higher binding properties to human serum albumin in comparison with catechin and conventional treatments. Consequently, it was assumed that the ultra-pressure treatment is an effective method for the extraction of bioactive compounds from indigo leaves.

• 出版日期2014-10