A coacervate-forming biodegradable polyester with elevated LCST based on bis-(2-methoxyethyl) amine

作者：Swanson J P; Martinez M R; Cruz M A; Mankoci S G; Costanzo P J; Joy A^*

来源：Polymer Chemistry, 2016, 7(28): 4693-4702.

DOI：10.1039/c6py00814c

Recently, we reported a new class of biodegradable, thermoresponsive polyesters (TR-PEs) inspired by polyacrylamides and elastin-like peptides (ELPs). The polyesters exhibit tunable cloud point temperatures (T-cp) and thermoresponsive coacervation in aqueous solution as shown via UV-vis spectroscopy, H-1 NMR, and DLS. However, the T-cp of all TR-PEs remained low (<15 degrees C), and higher thermoresponsivity would be beneficial for many applications. This study examines the synthesis, polymerization, and analysis of a new monomer bearing a more hydrophilic pendant group, bis-2-methoxyethylamine (bMoEtA). The resulting TR-PE, TR-bMoEtAPE, displays a threefold increase in T-cp (ca. 50 degrees C) that is affected by solution (DI water vs. phosphate buffered saline), concentration (1-40 mg mL(-1)) molecular weight (20-130 kDa), and cosolutes (Hofmeister salts and urea). The T-cp and T-g of random TR-bMoEtAPE copolymers can be tuned via comonomer feed. Variable temperature 1H NMR indicated a cooperative coacervation mechanism above T-cp, further reinforced by DLS measurements. As evidenced by UV-vis and SEC analysis, TR-bMoEtAPE underwent rapid degradation over a period of 7 days in DI water and PBS. Finally, cytotoxicity studies suggested that TR-bMoEtAPE is non-cytotoxic even at high concentrations (ca. 1000 mu g mL(-1)). The increased T-cp and tunability suggests TR-bMoEtAPE as a potential candidate for future functionalized TR-PE therapeutic-delivery systems.

• 出版日期2016