Degradation of Phytate Pentamagnesium Salt by Bacillus sp T4 Phytase as a Potential Eco-friendly Feed Additive

作者：Park Inkyung; Lee Jaekoo; Cho Jaiesoon^*

来源：Asian-Australasian Journal of Animal Sciences, 2012, 25(10): 1466-1472.

DOI：10.5713/ajas.2012.12276

• Phytase
• Bacillus sp.
• Magnesium Phytate
• Feed Additive
• Phosphorus Pollution

A bacterial isolate derived from soil samples near a cattle farm was found to display extracellular phytase activity. Based on 16S rRNA sequence analysis, the strain was named Bacillus sp. T4. The optimum temperature for the phytase activity toward magnesium phytate (Mg-InsP(6)) was 40 degrees C without 5 mM Ca2+ and 50 degrees C with 5 mM Ca2+. T4 phytase had a characteristic bi-hump two pH optima of 6.0 to 6.5 and 7.4 for Mg-InsP(6). The enzyme showed higher specificity for Mg-InsP(6) than sodium phytate (Na-InsP(6)). Its activity was fairly inhibited by EDTA, Cu2+, Mn2+, Co2+, Ba2+ and Zn2+. T4 phytase may have great potential for use as an eco-friendly feed additive to enhance the nutritive quality of phytate and reduce phosphorus pollution.

• 出版日期2012-10