A novel method for capillary electrophoresis directly analyzing trace amounts analytes in the water-immiscible solution samples

作者：Fang HF; Zeng ZR^*; Liu L; Pang DW

来源：Chemical Journal of Chinese Universities, 2006, 27(5): 856-858.

• solvent microextraction
• on-line back-extraction
• field amplified sample injection

This paper describes a novel method in which solvent microextraction (SME) is followed by on-line back-extraction-field amplified sample injection(OLBE-FASI) in capillary zone electrophoresis (CZE). The water-immiscible solution sample, sealed with water plug in the sample vial, was used for direct electroinjection. The water plug with moderate. content of organic solvent, low-conductivity, and the presence of a small amount of H+ provided the highest sensitivity for analyzing positively chargeable compounds, such as cocaine and thebaine. The linear range was 0.040-4, 0.040-2 mu g/mL, with the square of the correlation coefficient (r(2)) > 0.997, respectively. Detection limits were in the range of 5-10 ng/mL.

• 出版日期2006-5-10
• 单位武汉大学