Microfluidic reactor for lipase-catalyzed regioselective synthesis of neohesperidin ester derivatives and their antimicrobial activity research

作者：Du, Lihua^*; Jiang, Zhipeng; Xu, Liangliang; Zhou, Nani; Shen, Jiahong; Dong, Zhen; Shen, Le; Wang, Hong; Luo, Xiping

来源：Carbohydrate Research, 2018, 455: 32-38.

DOI：10.1016/j.carres.2017.11.008

• Neohesperidin
• Enzymatic synthesis
• Microreactor
• Lipases
• Regioselectivity

Lipase-catalyzed regioselective synthesis of neohesperidin ester derivatives was performed by Lipase TL IM from Thermomyces lanuginosus in a continuous-flow microreactor and then their antimicrobial activity was studied. It appears that neohesperidin, neohesperidin dihydrochalcone with primary OH on the sugar part is the most reactive substrate. Various reaction parameters were investigated including substrate molar ratio, reaction time and temperature. Maximum conversion (92%) was obtained under the optimal condition of substrate molar ratio of 8:1 (vinyl esters: neohesperidin) at 52 degrees C for about 35 min. Then, the antibacterial activity of modified neohesperidin ester derivatives was examined and showed great improvement against gram negative and gram positive bacteria.

• 出版日期2018-1-2
• 单位浙江工业大学