A self-assembled nano-cluster complex based on cytochrome c and nafion: An efficient nanostructured peroxidase

作者：Hong Jun^*; Wang Wei; Huang Kun; Yang Wei Yun; Zhao Ying Xue; Xiao Bao Lin; Gao Yun Fei; Moosavi Movahedi Zainab; Ahmadian Shahin; Bohlooli Mousa; Saboury Ali Akbar; Ghourchian Hedayatollah; Sheibani Nader; Moosavi Movahedi Ali Akbar

来源：Biochemical Engineering Journal, 2012, 65: 16-22.

DOI：10.1016/j.bej.2012.03.012

• Cytochrome c
• Nafion
• Nano-cluster
• Nanostructured peroxidase
• Self-assembled
• Highly efficient

A nano-cluster complex with highly efficient peroxide activity was constructed by self-assembly of nafion (NF) and cytochrome c (Cyt c) in 50 mM phosphate buffer at 25 degrees C. The UV-vis spectrometry, circular dichroism (CD) and transmission electron microscopy (TEM) methods were utilized for additional characterization of the nanostructured enzyme or artificial peroxidase (AP). The nano-cluster was composed of Chain-Ball structure, with an average ball size of approximately 40 nm detected by TEM method. The Michaelis-Menten (K-m) and catalytic rate (k(cat)) constants of the AP were obtained in 50 mM phosphate buffer solution at pH 7.0 which are equal to 2.5 +/- 0.4 mu M and 0.069 +/- 0.001 s(-1) respectively. The catalytic efficiency of AP was evaluated to be 0.028 +/- 0.005 mu M-1 s(-1), which was 39 +/- 5% as efficient as the native HRP. The AP generated here can be used in place of HRP with high stability and activity.

• 出版日期2012-6-15
• 单位河南大学