Methionine plus cystine levels and vitamin B-6 supplementation on performance and enzyme expression of methionine metabolism of gilts from 75 to 100 kg

作者：Sangali Cleiton Pagliari^*; Gasparino Eliane; Vasconcellos Ricardo Souza; Fachinello Marcelise Regina; Tricia Rigo Monteiro Alessandra Nardina; Costa Esteves Lucas Antonio; Bonagurio Lucas Pimentel; Pozza Paulo Cesar

来源：Brazilian Journal of Animal Science, 2017, 46(3): 223-230.

DOI：10.1590/S1806-92902017000300007

• blood variable
• cystathionine-gamma-lyase
• homocysteine
• methionine synthase
• performance
• pigs

This study was carried out to evaluate the effect of different levels of standardized ileal digestible (SID) methionine + cystine (Met+Cys) and vitamin B-6 supplementation on the performance, blood variables, and gene expression of enzymes involved in methionine metabolism in female pigs between 75 and 100 kg. Fifty six female pigs were used (Talent x Topigs 20), averaging 75.06 +/- 1.68 kg in initial weight, allotted in a completely randomized block design arranged in a 2 x 4 factorial scheme, composed of two vitamin B-6 supplementation levels (1.58 and 3.58 mg/kg) and four levels of SID Met+ Cys (0.370, 0.470, 0.570, and 0.670%), with seven replicates and one animal per experimental unit. No interactions between vitamin B6 supplementation and SID Met+ Cys levels were observed. The levels of SID Met+ Cys and vitamin B-6 supplementation did not affect animal performance. Triacylglycerols showed a quadratic response to the SID Met+ Cys levels, in which the lowest plasma concentration was estimated as 0.575%. Treatments did not affect the expression of the methionine synthase and cystathionine-gamma-lyase enzymes or serum homocysteine levels. The SID Met+ Cys requirement for female pigs from 75 to 100 kg is equal to or lower than 10.60 g/day, which corresponds to the level of 0.370% Met+Cys in the diet and a relationship 0.48% with the SID lysine.

• 出版日期2017-3