Development and validation of I-DNA1: a 15-Loci multiplex system for identity testing

作者：Odriozola A; Aznar J M; Celorrio D; Bravo M L; Builes J J; Val Bernal J F; de Pancorbo Marian M^*

来源：International Journal of Legal Medicine, 2011, 125(5): 685-694.

DOI：10.1007/s00414-010-0539-0

• Forensic science
• Mini and midi short tandem repeat
• STR
• DNA typing
• Degraded
• Multiplex
• DHPLC
• CODIS

This paper presents a system for the multiplex amplification of 15 loci, known as I-DNA1, which combines mini and midiSTR technology, with amplicon sizes ranging from 49 to 297 bp. I-DNA1 analyses all the STR loci included in the CODIS and the Interpol Standard Set of loci, nine of the ten European core loci and seven of the eight German core loci, making it suitable for use in identifying humans. Moreover, its high sensitivity and the small size of its amplicons mean that I-DNA1 is potentially highly useful for analysing highly degraded and/or very small DNA samples.

• 出版日期2011-9