Alumina sol-gel/sonogel-carbon electrode based on acetylcholinesterase for detection of organophosphorus pesticides

作者：Zejli Hanane; Hidalgo Hikdalgo de Cisneros Jose Luis; Naranjo Rodriguez Ignacio; Liu Baohong; Temsamani Khalid Riffi; Marty Jean Louis^*

来源：Talanta, 2008, 77(1): 217-221.

DOI：10.1016/j.talanta.2008.06.010

• sonogel-carbon electrode
• Enzymatic inhibition
• Organophosphorus pesticides

Two new amperometric biosensors based on immobilization of acetylcholinesterase on a sonogel-carbon electrode for detection of organophosphorous compounds are proposed. The electrodes were prepared applying high-energy ultrasounds directly to the precursors. The first biosensor was obtained by simple entrapping acetylcholinesterase in Al2O3 sol-gel matrix on the sonogel-carbon. The second biosensor was produced in a sandwich configuration. Its preparation involved adsorption of the enzyme and modification via a polymeric membrane such as polyethylene glycol and the ion-exchanger Nafion.The optimal enzyme loading was found to be 0.7 mIU. Both biosensors showed optimal activity in 0.2 M phosphate buffer, pH 7.0, at an operating potential of 210 mV. The detection limit achieved for chlorpyriphos-ethyl-oxon was 2.5 x 10(-10) M at a 10-min incubation time.

• 出版日期2008-10-15
• 单位复旦大学