Acute hyperglycaemia does not alter nitric oxide-mediated microvascular function in the skin of adolescents with type 1 diabetes

作者：Naylor Louise H^*; Yusof Norhaida M; Paramalingam Nirubasini; Jones Timothy W; Davis Elizabeth A; Green Daniel J

来源：European Journal of Applied Physiology, 2014, 114(2): 435-441.

DOI：10.1007/s00421-013-2785-6

• Blood glucose
• Microvessel
• Skin blood flow
• Endothelial function
• Diabetes
• Adolescent

Purpose We assessed the impact of an acute bout of hyperglycaemia on nitric oxide (NO)-mediated microvascular function in the skin of adolescents with type 1 diabetes (T1DM). %26lt;br%26gt;Methods Twelve subjects (12-18 years) with T1DM were randomised into a control (n = 6) or hyperglycaemia (n = 6) group. Hyperinsulinaemic clamps were used to manipulate blood glucose level (BGL). Following a baseline period, where all subjects were euglycaemic (20 min), the experimental phase began. During the experimental phase BGL was elevated to 16.7 +/- 0.9 mmol L-1 in the hyperglyceamic group, while it was maintained at euglycaemia (5.5 +/- 0.1 mmol L-1) in the control group. Simultaneously, cutaneous microvascular % max cutaneous vascular conductance, CVC%) was assessed using laser Doppler fluxometry following stimulation of skin blood flow using localised heating (42 degrees C). To determine the NO contribution to skin blood flow, two microdialysis sites were assessed, one perfused with Ringers and the other with the NO blocker, NG-monomethyl-L-arginine (L-NMMA). %26lt;br%26gt;Results In the hyperglycaemic group, acute increase in BGL was not associated with changes in skin blood flow (CVC% 82.4 +/- 8.7 % at 5.5 +/- 0.1 mmol L-1 vs 79.5 +/- 9.1 % at 16.7 +/- 0.9 mmol L-1, unpaired t tests, P = 0.588) or the contribution of NO to vasodilation. %26lt;br%26gt;Conclusions These results suggest that, in our group of adolescents with type 1 diabetes, acute hyperglycaemia did not affect skin microvascular NO-mediated function.

• 出版日期2014-2