A Search for Hepatoprotective Activity of Fruit Extract of Mangifera indica L. Against Oxidative Stress Cytotoxicity

作者：Pourahmad Jalal^*; Eskandari Mohammad Reza; Shakibaei Rashin; Kamalinejad Mohammad

来源：Plant Foods for Human Nutrition, 2010, 65(1): 83-89.

DOI：10.1007/s11130-010-0161-9

• Hepatoprotection
• Lysosomes
• Mangifera indica L.
• Mitochondria
• Oxidative stress

Mango (Mangifera indica L.) and their components are commonly used in folk medicine for many curative effects. The protective effects of different concentrations of aqueous extract of Mangifera indica L. fruit (Mango Extract) (20, 50 and 100 mu g/ml) and also gallic acid (100 mu M) as a pure compound in the extract were examined against oxidative stress toxicity induced by cumene hydroperoxide (CHP) in isolated rat hepatocytes. The extracts and gallic acid (100 mu M) protected the hepatocyte against all oxidative stress markers including cell lysis, ROS generation, lipid peroxidation, glutathione depletion, mitochondrial membrane potential decrease, lysosomal membrane oxidative damage and cellular proteolysis. Mango Extracts (20, 50 and 100 mu g/ml) were more effective than gallic acid (100 mu M) in protecting hepatocytes against CHP induced lipid peroxidation. On the other hand gallic acid (100 mu M) acted more effective than Mango Extracts (20, 50 and 100 mu g/ml) at preventing lysosomal membrane damage. In addition H(2)O(2) scavenging effect of all extracts were determined in hepatocytes and compared with gallic acid (100 mu M). There were no significance differences (P < 0.05) between all plant extracts and gallic acid (100 mu M) in H(2)O(2) scavenging activity. These results suggest a hepatoprotective role for Mango Extract against liver injury associated with oxidative stress.

• 出版日期2010-3