Alpha lipoic acid selectively inhibits proliferation and adhesion to fibronectin of v-H-ras-transformed 3Y1 cells

作者：Yamasaki Masao; Iwase Masahiro; Kawano Kazuo; Sakakibara Yoichi; Suiko Masahito; Nishiyama Kazuo^*

来源：Journal of Clinical Biochemistry and Nutrition, 2012, 50(3): 234-240.

DOI：10.3164/jcbn.11-67

• reactive oxygen species
• beta 1-integrin
• abnormal growth
• 3Y1 cells

Here, we focused on the effects of racemic a-lipoic acid on proliferation and adhesion properties of 3Y1 rat fibroblasts and the v-H-ras-transformed derivative, HR-3Y1-2 cells. Racemic a-lipoic acid inhibited proliferation of HR-3Y1-2 but not 3Y1 cells at 0.3 and 1.0 mM. R-(+)-alpha-lipoic acid also inhibited proliferation of HR-3Y1-2 cells equivalent to that of racemic a-lipoic acid. In addition, racemic a-lipoic acid decreased intracellular reactive oxygen species levels in HR-3Y1 cells but not 3Y1 cells. Next, we evaluated the effects of racemic a-lipoic acid on cell adhesion to fibronectin. The results indicated that racemic a-lipoic acid decreased adhesive ability of HR-3Y1-2 cells to fibronectin-coated plates. As blocking antibody experiment revealed that beta 1-integrin plays a key role in cell adhesion in this experimental system, the effects of racemic a-lipoic acid on the expression of beta 1-integrin were examined. The results indicated that racemic a-lipoic acid selectively down-regulated the expression of cell surface beta 1-integrin expression in HR-3Y1-2 cells. Intriguingly, exogenous hydrogen peroxide upregulated cell surface beta 1-integrin expression in 3Y1 cells. Taken together, these data suggest that reduction of intracellular reactive oxygen species levels by a-lipoic acid could be an effective means of ameliorating abnormal growth and adhesive properties in v-H-ras transformed cells.

• 出版日期2012-5-1