Compartment size dependence of performance of polymerase chain reaction inside giant vesicles

作者：Shohda Koh ichiroh^*; Tamura Mieko; Kageyama Yoshiyuki; Suzuki Kentaro; Suyama Akira; Sugawara Tadashi

来源：Soft Matter, 2011, 7(8): 3750-3753.

DOI：10.1039/c0sm01463j

The amplification of a 1229 bp template DNA that encoded the green fluorescent protein (GFP) was conducted in cell-sized giant vesicles (GVs, phi > 1 mu m) using a real-time polymerase chain reaction (PCR) technique. The proportion of PCR-proceeded GV reached up to 16% of all GVs. The dependence of PCR on GV size was elucidated by flow cytometry analysis.

• 出版日期2011