Next-generation sequencing to generate interactome datasets

作者:Yu Haiyuan; Tardivo Leah; Tam Stanley; Weiner Evan; Gebreab Fana; Fan Changyu; Svrzikapa Nenad; Hirozane Kishikawa Tomoko; Rietman Edward; Yang Xinping; Sahalie Julie; Salehi Ashtiani Kourosh; Hao Tong; Cusick Michael E; Hill David E; Roth Frederick P; Braun Pascal*; Vidal Marc
来源:Nature Methods, 2011, 8(6): 478-U2257.
DOI:10.1038/NMETH.1597

摘要

Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCRCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.

  • 出版日期2011-6