An injectable cell penetrable nano-polyplex hydrogel for localized siRNA delivery

作者：Kim Young Min; Park Mi Ran; Song Soo Chang^*

来源：Biomaterials, 2013, 34(18): 4493-4500.

DOI：10.1016/j.biomaterials.2013.02.050

• Cell penetration
• Gene delivery
• Nano-polyplex
• Polyplex hydrogel
• Small interference RNA (siRNA)

An approach for application of cell penetration to selective small interference RNA (siRNA) localized delivery system, cell penetrable nano-polyplex assembled hydrogel system, is presented. The cell penetrable nano-polyplex assembled hydrogelisprepared by protamine conjugation to poly(organophosphazene) and inducement of nano-polyplexes with siRNAs. After an injection of cell penetrable nano-polyplex solution into the body, it turns into a gel due to thermosensitivity of poly(organophosphazene). The gel maintains up to 4 weeks and the released 30 nm-sized nano-polyplexes from the gel induces highly effective siRNA delivery due to cell penetration. Accordingly, the new system shows a high gene silencing efficiency on only the target site in long-term with a single injection.

• 出版日期2013-6