Acteoside from Cistanche salsa inhibits apoptosis by 1-methyl-4-phenylpyridinium ion in cerebellar granule neurons

作者：Pu XP^*; Song ZH; Li YY; Tu PF; Li HN

来源：Planta Medica, 2003, 69(1): 65-66.

DOI：10.1055/s-2003-37029

In this study we assessed the effect of acteoside that significantly improved cell viability and inhibited lactate dehydrogenase (LDH) release. Furthermore acteoside prevented a neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+)-induced apoptosis in CGNs. Accordingly, our flow cytometric analysis of CGNs after acteoside treatment revealed a decrease in the number of the MPP+-induced apoptotic cells (P < 0.001). Western blot analysis demonstrated that acteoside inhibits the active caspase-3 fragment (17 kDa) (P < 0.001) and the proteolytic poly (ADP-ribose) polymerase (PARP) fragment (85 kDa) expression (P < 0.001) following MPP+ treatment in CGNs. We conclude that acteoside prevents the MPP+-induced apoptosis and inhibits the apoptosis-related pathway.

• 出版日期2003-1
• 单位北京大学