ERES: sites for autophagosome biogenesis and maturation?

作者：Sanchez Wandelmer Jana; Ktistakis Nicholas T^*; Reggiori Fulvio

来源：Journal of Cell Science, 2015, 128(2): 185-192.

DOI：10.1242/jcs.158758

• Phagophore assembly site
• Pre-autophagosomal membrane
• Phagophore
• Isolation membrane
• Autophagosome
• Autophagy
• Endoplasmic reticulum exit site

Autophagosomes are the hallmark of autophagy, but despite their central role in this degradative pathway that involves vesicle transport to lysosomes or vacuoles, the mechanism underlying their biogenesis still remains largely unknown. Our current concepts about autophagosome biogenesis are based on models suggesting that a small autonomous cisterna grows into an autophagosome through expansion at its extremities. Recent findings have revealed that endoplasmic reticulum (ER) exit sites (ERES), specialized ER regions where proteins are sorted into the secretory system, are key players in the formation of autophagosomes. Owing to the morphological connection of nascent autophagosomes with the ER, this has raised several questions that challenge our current perception of autophagosome biogenesis, such as are ERES the compartments where autophagosome formation takes place? What is the functional relevance of this connection? Are these compartments providing essential molecules for the generation of autophagosomes and/or are they structural platforms where these vesicles emerge? In this Hypothesis, we discuss recent data that have implicated the ERES in autophagosome biogenesis and we propose two models to describe the possible role of this compartment at different steps in the process of autophagosome biogenesis.

• 出版日期2015-1-15