Diffraction phase microscopy: retrieving phase contours on living cells with a wavelet-based space-scale analysis

作者：Martinez Torres Cristina; Berguiga Lotfi; Streppa Laura; Boyer Provera Elise; Schaeffer Laurent; Elezgaray Juan; Arneodo Alain; Argoul Francoise^*

来源：Journal of Biomedical Optics, 2014, 19(3): 036007.

DOI：10.1117/1.JBO.19.3.036007

• quantitative phase imaging
• diffraction phase microscopy
• continuous wavelet transform
• space-scale analysis
• interferometry

We propose a two-dimensional (2-D) space-scale analysis of fringe patterns collected from a diffraction phase microscope based on the 2-D Morlet wavelet transform. We show that the adaptation of a ridge detection method with anisotropic 2-D Morlet mother wavelets is more efficient for analyzing cellular and high refractive index contrast objects than Fourier filtering methods since it can separate phase from intensity modulations. We compare the performance of this ridge detection method on theoretical and experimental images of polymer microbeads and experimental images collected from living myoblasts. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

• 出版日期2014-3