Comparative morphology and phagocytic capacity of primary human adult microglia with time-lapse imaging

作者：Levtova Natalie; Healy Luke M; Marysol Catalina; Gonczi Carvajal; Stopnicki Brandon; Blain Manon; Kennedy Timothy E; Moore Craig S; Antel Jack P; Darlington Peter J^*

来源：Journal of Neuroimmunology, 2017, 310: 143-149.

DOI：10.1016/j.jneuroim.2017.05.012

• Primary human adult microglia
• Dendritic cells
• E. coli bioparticles
• Time lapse microscopy
• Pseudopodic
• Ramified
• Amoeboid

Microglia provide immune surveillance within the brain and spinal cord. Various microglial morphologies include ramified, amoeboid, and pseudopodic. The link between form and function is not clear, especially for human adult microglia which are limited in availability for study. Here, we examined primary human microglia isolated from normal-appearing white matter. Pseudopodic and amoeboid microglia were effective phagocytes, taking up E. coli bioparticles using ruffled cell membrane sheets and retrograde transport. Pseudopodic and amoeboid microglia were more effective phagocytes as compared to ramified microglia or monocyte-derived dendritic cells. Thus, amoeboid and pseudopodic microglia may both be effective as brain scavengers.

• 出版日期2017-9-15