Beyond Identification: Emerging and Future Uses for MALDI-TOF Mass Spectrometry in the Clinical Microbiology Laboratory

作者：DeMarco Mari L; Ford Bradley A^*

来源：Clinics in Laboratory Medicine, 2013, 33(3): 611-+.

DOI：10.1016/j.cll.2013.03.013

• Antimicrobial susceptibility testing
• Bacteremia
• Bloodstream infection
• Direct identification
• MALDI-TOF
• Mass spectrometry
• Urinary tract infection
• Machine learning

The routine use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microorganism identification in the clinical microbiology laboratory. Building from these now common microorganism identification strategies, this review explores future clinical applications of MALDI-TOF MS. This includes practical approaches for laboratorians interested in implementing direct identification processing methods for MALDI-TOF detection of microbes in bloodstream infection (BSI) and urinary tract infection (UTI), as well as, post-analytical approaches for classifying MALDI-TOF spectral data to detect characteristics other and species-level identification (e.g. strain-level classification, typing, and resistance mechanisms).

• 出版日期2013-9