Affinity-mass spectrometric technologies for quantitative proteomics in biological fluids

作者：Li Huiyan; Popp Robert; Borchers Christoph H^*

来源：TrAC-Trends in Analytical Chemistry, 2017, 90: 80-88.

DOI：10.1016/j.trac.2017.02.011

• Protein quantification
• Biomarkers
• Affinity-mass spectrometry
• Immuno-enrichment
• Limit of detection
• Specificity
• Multiplexing
• Throughput

Proteins are the functional molecules in organisms and are therefore excellent biomarker candidates for a diversity of diseases. Immunoassays and mass spectrometry (MS) are two major technologies being used in proteomics; however, they either lack specificity or sensitivity. An emerging trend is to combine immunoassays with MS (which we call "affinity-MS"). This is an important milestone in quantitative proteomics, making it possible to measure low-abundance proteins with high specificity. The targeted enrichment and the assignment of mass-to-charge ratios to different molecules provide two selection criteria, making affinity-MS highly specific. Picogram-per-milliliter limits of detection have been obtained for many proteins. Furthermore, multiplexing capacity of >150 proteins has been achieved. This article reviews different formats of affinity-enrichment methods, and demonstrates how they are interfaced with both electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) MS. The pros and cons of these techniques are compared, and future prospectives are discussed.

• 出版日期2017-5