Mechanism of macrophage activation by (1,4)-alpha-D-glucan isolated from Tinospora cordifolia

作者：Nair P K Raveendran; Melnick Steven J; Ramachandran Reshma; Escalon Enrique; Ramachandran Cheppail^*

来源：International Immunopharmacology, 2006, 6(12): 1815-1824.

DOI：10.1016/j.intimp.2006.07.028

• immunostimulation
• macrophages
• toll-like receptor
• TNF-alpha
• NF-kappa B

The signaling mechanism of the novel (1,4)-alpha-D-glucan (RR1) isolated from the medicinal plant Tinospora cordifolia was investigated in macrophages to evaluate its immunostimulating properties. When RAW264.7 macrophages were incubated with RR1 at 4 degrees C, the novel glucan inhibited the phagocytosis of unopsonized zymosan A bioparticles in a dose-dependent manner. RR1 also inhibited the binding and internalization of opsonized zymosan A bioparticles, although at a lower level than laminarin. Incubation of macrophages with anti-CD11b mAb followed by RR1 failed to show any inhibitory effect on RR1-induced TNF-alpha synthesis confirming that complement receptor 3 (CR3) is not involved in the opsonic binding and internalization of RR1 in macrophages unlike zymosan A. The anti-CD11b mAb has significant inhibitory effect on the zymosan A-induced tumor necrosis factor (TNF)-alpha synthesis. RR1 induced TNF-a synthesis in macrophages in a dose-dependent manner which can be completely inhibited by the NF-kappa B inhibitor caffeic acid phenethyl ester (CAPE) or curcumin. RR1 activated NF-KB in a time- and dose-dependent manner and this modulation of nuclear NF-KB activity is associated with the degradation of I-kappa B a thus facilitating the translocation of NF-KB into the nucleus. RR1-induced NF-KB activity peaks at 8 h of RR1 stimulation while I-kappa B a degradation occurred within I h of stimulation. RR1-induced NF-KB activation occurred through TLR6 signaling as evidenced by the synthesis of IL-8 in TLR6-transfected HEK293 cells. These results show that the novel (1,4)-a-D-glucan from Tinospora cord folia activates the immune system through the activation of macrophages that occurs through TLR6 signaling, NF-KB translocation and cytokine production.

• 出版日期2006-12-5