Simultaneous determination of 41 components in Gualou Guizhi granules by UPLC coupled with triple quadrupole mass spectrometry

作者：Lin Yu; Xu Wei; Xu Wen; Huang Mingqing; Zhang Yuqin; Li Huang; Chu Kedan; Chen Lidian^*

来源：Analytical Methods, 2015, 7(19): 8285-8296.

DOI：10.1039/c5ay01336d

Gualou Guizhi granules (GLGZGs) are a classical formula of traditional Chinese medicine, which have been commonly used to treat dysfunction after stroke, epilepsy and spinal cord injury. In this study, an efficient method using ultra-performance liquid chromatography (UPLC) coupled with triple quadrupole mass spectrometry (QqQ MS) was developed for the rapid determination of forty-one major components in GLGZGs. Chromatographic separation was achieved on a Waters ACQUITY UPLC Cortest C18 column (100 mm x 2.1 mm, 1.6 mu m) with a gradient mobile phase (A: 0.1% aqueous formic acid and B: acetonitrile with 0.1% formic acid) at a flow rate of 0.25 mL min(-1). Multiple-reaction monitoring (MRM) was used for quantitative analysis. Ten batches of GLGZGs were analyzed with a good linear regression relationship (R-2, 0.9841-0.9998). The present study offered highly sensitive, specific and speedy determination of forty-one components, which promoted the quality control investigation of GLGZGs via employing the developed method.

• 出版日期2015
• 单位福建中医药大学