Anti-nociceptive mechanisms of flavonoids-rich methanolic extract from Terminalia coriacea (Roxb.) Wight & Arn. leaves

作者：Khan Mohammed Safwan Ali; Ahmed Nishat; Mi**ah; Arifuddin Mohammed; Zakaria Zainul Amiruddin; Al Sanea Mohammad M; Khundmiri Syeda Umme Kulsoom; Ahmed Inshah; Ahmed Saleha; Mok Pooi Ling

来源：Food and Chemical Toxicology, 2018, 115: 523-531.

DOI：10.1016/j.fct.2018.03.021

• Terminalia coriacea
• Flavonoids
• Anti-nociceptive mechanisms
• Opioid
• Nitric oxide
• ATP sensitive K plus channel

In view of the report on anti-nociceptive activity of Leathery Murdah, Terminalia coriacea {Roxb.} Wight & Am. (Combretaceae) leaves, the present study was conducted to isolate the active constituents and identify the underlying mechanisms. The methanolic extract of T. coriacea leaves (TCLME) at doses 125, 250 and 500 mg/kg orally, was subjected to various in-vivo assays in acetic acid induced writhing and formalin induced paw-licking tests with aspirin (100 mg/kg) and morphine (5 mg/kg) as reference drugs. Three flavonoids, rutin, robinin and gossypetin 3-glucuronide 8-glucoside were isolated and characterized from TCLME for the first time. The extract showed significant (p < 0.001) dose-dependent anti-nociceptive activity in glutamate induced paw licking in mice. The involvement of opioid pathway was confirmed as naloxone (5 mg/kg, i.p) treatment blocked the analgesic activity of the test extract. Similarly, glibenclamide (an ATP - sensitive potassium channel inhibitor) at dose of 10 mg/kg, i.p increased writhing in acetic acid model. It reversed the inhibitory effects of TCLME when administered in combination. Treatment of TCLME alone and in combination with L-arginine (100 mg/kg, i.p) significantly (p < 0.001) reduced writhing while pre-treatment with L-NAME (20 mg/kg, i.p) further enhanced the analgesic action of TCLME indicating involvement of nitric oxide pathway.

• 出版日期2018-5