D-beta-Hydroxybutyrate inhibited the apoptosis of PC12 cells induced by H2O2 via inhibiting oxidative stress

作者：Cheng Baohua^*; Lu Hai; Bai Bo; Chen Jing

来源：Neurochemistry International, 2013, 62(5): 620-625.

DOI：10.1016/j.neuint.2012.09.011

• D-beta-Hydroxybutyrate
• Apoptosis
• PC12
• H2O2
• Oxidative stress

Oxidative stress has an important role in neurodegenerative diseases and cerebral ischemic injury. It is reported that D-beta-hydroxybutyrate (D beta HB), the major component of ketone bodies, is neuroprotective in recent studies. Therefore, in the present work the neuroprotective effects of D beta HB on H2O2-induced apoptosis mediated by oxidative stress was investigated. PC12 cells were exposed to H2O2 with different concentrations of H2O2 for different times after D beta HB pretreatment. MTT assay, apoptotic rates, intracellular reactive oxygen species (ROS) level, GSH content, mitochondrial membrane potential (MMP) and caspase-3 activity were determined. The results showed that D beta HB inhibited the decrease of cell viability induced by H2O2 in PC12 cells. D beta HB decreased the apoptotic rates induced by H2O2. The changes of intracellular ROS, GSH, MMP and caspase-3 activity due to H2O2 exposure were partially reversed in PC12 cells. So D beta HB inhibited the apoptosis of PC12 cells induced by H2O2 via inhibiting oxidative stress.

• 出版日期2013-4
• 单位济宁医学院