Uptake of raft components into amyloid beta-peptide aggregates and membrane damage

作者：Sasahara Kenji^*; Morigaki Kenichi; Mori Yasuko

来源：Analytical Biochemistry, 2015, 481: 18-26.

DOI：10.1016/j.ab.2015.04.014

• Amyloid beta-peptide
• Amyloid aggregation
• Model membrane
• Membrane heterogeneity
• Lipid raft

Amyloid aggregation and deposition of amyloid beta-peptide (A beta) are pathologic characteristics of Alzheimer's disease (AD). Recent reports have shown that the association of A beta with membranes containing ganglioside GM1 (GM 1) plays a pivotal role in amyloid deposition and the pathogenesis of AD. However, the molecular interactions responsible for membrane damage associated with A beta deposition are not fully understood. In this study, we microscopically observed amyloid aggregation of A beta in the presence of lipid vesicles and on a substrate-supported planar membrane containing raft components and GM1. The experimental system enabled us to observe lipid-associated aggregation of A beta, uptake of the raft components into A beta aggregates, and relevant membrane damage. The results indicate that uptake of raft components from the membrane into A beta deposits induces macroscopic heterogeneity of the membrane structure.

• 出版日期2015-7-15