Detection of Extended Spectrum Beta-Lactamases Resistance Genes among Bacteria Isolated from Selected Drinking Water Distribution Channels in Southwestern Nigeria

作者：Adesoji Ayodele T; Ogunjobi Adeniyi A

来源：Biomed Research International, 2016, 2016: 7149295.

DOI：10.1155/2016/7149295

<jats:p>Extended Spectrum Beta-Lactamases (ESBL) provide high level resistance to beta-lactam antibiotics among bacteria. In this study, previously described multidrug resistant bacteria from raw, treated, and municipal taps of DWDS from selected dams in southwestern Nigeria were assessed for the presence of ESBL resistance genes which include<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">E</mml:mi><mml:mi mathvariant="normal">M</mml:mi></mml:mrow></mml:msub></mml:math>,<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">S</mml:mi><mml:mi mathvariant="normal">H</mml:mi><mml:mi mathvariant="normal">V</mml:mi></mml:mrow></mml:msub></mml:math>, and<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">C</mml:mi><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">X</mml:mi></mml:mrow></mml:msub></mml:math>by PCR amplification. A total of 164 bacteria spread across treated (33), raw (66), and municipal taps (68), belonging to<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mrow><mml:mi>α</mml:mi></mml:mrow></mml:math>-Proteobacteria,<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mrow><mml:mi>β</mml:mi></mml:mrow></mml:math>-Proteobacteria,<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M6"><mml:mrow><mml:mi mathvariant="normal">γ</mml:mi></mml:mrow></mml:math>-Proteobacteria, Flavobacteriia, Bacilli, and Actinobacteria group, were selected for this study. Among these bacteria, the most commonly observed resistance was for ampicillin and amoxicillin/clavulanic acid (61 isolates). Sixty-one isolates carried at least one of the targeted ESBL genes with<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M7"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">E</mml:mi><mml:mi mathvariant="normal">M</mml:mi></mml:mrow></mml:msub></mml:math>being the most abundant (50/61) and<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M8"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">C</mml:mi><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">X</mml:mi></mml:mrow></mml:msub></mml:math>being detected least (3/61).<jats:italic>Klebsiella</jats:italic>was the most frequently identified genus (18.03%) to harbour ESBL gene followed by<jats:italic>Proteus</jats:italic>(14.75%). Moreover, combinations of two ESBL genes,<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M9"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">S</mml:mi><mml:mi mathvariant="normal">H</mml:mi><mml:mi mathvariant="normal">V</mml:mi></mml:mrow></mml:msub><mml:mo>+</mml:mo><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">E</mml:mi><mml:mi mathvariant="normal">M</mml:mi></mml:mrow></mml:msub></mml:math>or<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M10"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">C</mml:mi><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">X</mml:mi></mml:mrow></mml:msub><mml:mo>+</mml:mo><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">E</mml:mi><mml:mi mathvariant="normal">M</mml:mi></mml:mrow></mml:msub></mml:math>, were observed in 11 and 1 isolate, respectively. In conclusion, classic<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M11"><mml:mi>b</mml:mi><mml:mi>l</mml:mi><mml:msub><mml:mrow><mml:mi>a</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">T</mml:mi><mml:mi mathvariant="normal">E</mml:mi><mml:mi mathvariant="normal">M</mml:mi></mml:mrow></mml:msub></mml:math>ESBL gene was present in multiple bacterial strains that were isolated from DWDS sources in Nigeria. These environments may serve as foci exchange of genetic traits in a diversity of Gram-negative bacteria.</jats:p>

• 出版日期2016