Association analysis of hepatitis virus B infection with haplotypes of the TBX21 gene promoter region in the Chinese population

作者：Cao Bangwei; Yang Hongli; Ding Howard; Qi Suxia; Gao Lei; Cui Hong; Dai Yue; Xu Changqing^*

来源：Clinical Chemistry and Laboratory Medicine, 2007, 45(3): 333-338.

DOI：10.1515/CCLM.2007.062

• genetic susceptibility
• haplotype
• hepatitis B virus (HBV)
• promoter
• TBX21 (T-box21) gene

Background: The T-box21 (TBX21) gene encodes the transcription factor T-bet (T-box expressed in T-cells), which influences naive T-lymphocyte development and has been implicated in the pathogenesis of many diseases.
Methods: We selected 208 hepatitis B patients and 213 healthy volunteers to examine whether polymorphisms or haplotypes of the TBX21 gene promoter were associated with hepatitis B virus (HBV) infection in the Chinese population. Two polymorphisms at -1499 and -1514 located in the TBX21 promoter region were identified by the PCR-restriction fragment length polymorphism (PCR-RFLP) method.
Results: Single nucleoticle polymorphism (SNP) at -1499 was significantly different between HBV patients and healthy controls [p=0.003; odds ratio (OR) 3.65, 95% confidence interval (CI) 1.58-8.45]. Similarly, our results showed a significantly higher level of haplotype D (--/AC) in HBV patients compared to control subjects (p=0.005; OR 4.82, 95% Cl 1.59-14.61).
Conclusions: Based on our findings, it seems that genetic variations of allele -1499 and haplotype D (--/AC) within the TBX21 promoter region contribute to susceptibility to HBV infection in the Chinese population.

• 出版日期2007
• 单位北京大学