Determination of adrenolytic drugs by SPME-LC-MS

作者：Buszewski Boguslaw^*; Olszowy Pawel; Ligor Tomasz; Szultka Malgorzata; Nowaczyk Jacek; Jaworski Maciej; Jackowski Marek

来源：Analytical and Bioanalytical Chemistry, 2010, 397(1): 173-179.

DOI：10.1007/s00216-010-3483-4

• Adrenolytic drugs
• Solid-phase microextraction
• High-performance liquid chromatography
• Mass spectrometry

Five adrenolytic drugs have been analyzed by liquid chromatography-mass spectrometry (LC-MS). Samples were prepared by solid-phase microextraction (SPME) using polypyrrole fibers coated on stainless steel support as an adsorbent for the drugs. Adsorption efficiencies were 95% and were close for all the drugs investigated. Relative standard deviations (RSD), calculated for samples prepared in standard solutions, were in the range 2.5-13%, however RSD values for the drugs in human plasma were 2.5-4.5%. Using LC-MS the limit of detection (LOD) and the limit of quantification (LOQ) were in the ranges 0.11-0.18 and 0.39-0.54 ng mL(-1), respectively, for the five drugs.

• 出版日期2010-5