Analysis of Hydrolysis Products of Flavone Glycosides of Radix Astragali Using High Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

作者：Xiao Wei Hua^*; Han Lu Jia

来源：Chinese Journal of Analytical Chemistry, 2008, 36(10): 1399-1402.

• High performance liquid chromatography-electrospray ionization spectrometry
• beta-glucosidase
• flavone glycosides
• flavone aglycones
• radix astragali

The results analyzed using LC-MS indicated that, the main flavonoids contained in Radix Astragali are flavone beta-glycosides including calycosin-7-O-beta-D-glucoside, ononion, 9,10-di-methoxypterocarpan-3-O-beta-D-glucoside and 2';-dihydroxy-3';, 4';-dimethyl-isoflavan-7-O-beta-D-glucoside. beta-glucosidase was chosen for the hydrolysis of flavone glycosides in Radix Astragali, which effciently converted the flavone glycosides into aglycones including calycosin, formononetin, 3-hydroxy-9, 10-di-methoxypterocarpan and 7, 2';-dlhydroxy-3';,4';-dimethylisoflavan according to the LC-MS results. The hydrolysis rate of the flavone glycosides by beta-glucosidase was over 90%. The antioxidant activity of the hydrolyzed product of radix astragali was increased greatly through enzyme treatment which was almost 1.4 times comparing with that of the flavonoid extract without treatment.

• 出版日期2008-10
• 单位中国农业大学