Nine microsatellite markers for the Australian side-necked turtle Chelodina expansa (Chelidae) and cross species amplifications

作者：Baggiano Olivier^*; Schmidt Daniel James; Hughes Jane Margaret

来源：Marine Genomics, 2011, 4(4): 297-300.

DOI：10.1016/j.margen.2011.08.002

• Chelidae
• Primer
• Multiplex PCR
• Fluorescent
• Turtle
• Australia

Nine microsatellite DNA loci for the Australian broad-shelled freshwater turtle (Chelodina expansa) are presented. Markers were tailed with 20-met oligonucleotides for use in four-colour fluorescent multiplex PCRs. The markers show high allelic richness (mean N(A) = 10.9, range 2-38) and expected heretozygosity (mean H(E) = 0.643; range 0.161-0.963) indicating that they will be valuable for population genetics studies in C. expansa. Cross-species amplification in three Australian freshwater turtle species further highlights the potential utility of these markers, particularly in the side-neck species C. longicollis and C. rugosa.

• 出版日期2011-12