A Stepwise NaHSO3 Addition Mode Greatly Improves H-2 Photoproduction in Chlamydomonas reinhardtii

作者：Wei, Lanzhen; Li, Xin; Fan, Baoqiang; Ran, Zhaoxing; Ma, Weimin^*

来源：Frontiers in Plant Science, 2018, 9: 1532.

DOI：10.3389/fpls.2018.01532

• NaHSO3
• stepwise mode
• anaerobic environment
• PSII activity
• H-2 photoproduction
• Chlamydomonas reinhardtii

NaHSO3 addition greatly increases the yield of H-2 photoproduction in a unicellular green alga Chlamydomonas reinhardtii through removing O-2 and activating hydrogenase but significantly impairs the activity of PSII, an electron source for H-2 photoproduction. Here, a stepwise addition mode of total 13 mM NaHSO3, an optimal concentration for H-2 photoproduction of C. reinhardtii identified in a previous one step addition method, significantly improved H-2 photoproduction. Such improvement was believed to be the result of increased residual PSII activity in an anaerobic background, but was at least independent of two alternative electron sinks for H-2 photoproduction, cyclic electron transport around PSI and CO2 assimilation. Based on the above results, we propose that increased residual PSII activity in an anaerobic environment is an efficient strategy to enhance H-2 photoproduction in C. reinhardtii, and the stepwise NaHSO3 addition mode is a case study in the strategy.

• 出版日期2018-10-31
• 单位上海师范大学