An Na+/H+ antiporter gene from wheat plays an important role in stress tolerance

作者：Yu Jia Ning; Huang Han; Wang Zi Ning; Zhang Jin Song; Chen Shou Yi^*

来源：Journal of Biosciences, 2007, 32(6): 1153-1161.

DOI：10.1007/s12038-007-0117-x

• Na+/H+ antiporter
• stress tolerance
• transcription accumulation
• yeast cells
• wheat

A vacuole Na+/H+ antiporter gene TaNHX2 was obtained by screening the wheat cDNA library and by the 5'-RACE method. The expression of TaNHX2 was induced in roots and leaves by treatment with NaCl, polyethylene glycol (PEG), cold and abscisic acid (ABA). When expressed in a yeast mutant (Delta nhx1), TaNHX2 suppressed the salt sensitivity of the mutant, which was deficient in vacuolar Na+/H+ antiporter, and caused partial recovery of growth of Delta nhx1 in NaCl and LiCl media. The survival rate of yeast cells was improved by overexpressing the TaNHX2 gene under NaCl, KC1, sorbitol and freezing stresses when compared with the control. The results imply that TaNHX2 might play an important role in salt and osmotic stress tolerance in plant cells.

• 出版日期2007-9
• 单位中国科学院; 陕西师范大学