科研之友
微信
新浪微博
Facebook
分享链接
摘要
Recently, a plasma flow has been applied to medical treatment using effects of various kinds of stimuli such as chemical species, charged particles, heat, light, shock wave and electric fields. Among them, the chemical species are known to cause an inactivation of cell viability. However, the mechanisms and key factors of this event are not yet clear. In this study, we focused on the effect of H(2)O(2) in plasma-treated culture medium because it is generated in the culture medium and it is also chemically stable compared with free radicals generated by the plasma flow. To elucidate the significance of H(2)O(2), we assessed the differences in the effects of plasma-treated medium and H(2)O(2)-added medium against inactivation of HeLa cell viability. These two media showed comparable effects on HeLa cells in terms of the survival ratios, morphological features of damage processes, permeations of H(2)O(2) into the cells, response to H(2)O(2) decomposition by catalase and comprehensive gene expression. The results supported that among chemical species generated in a plasma-treated culture medium, H(2)O(2) is one of the main factors responsible for inactivation of HeLa cell viability.
- 出版日期2011-9-21
