Development of a Single-channel Multiple Immunoassay Chip

作者:Tanaka Masato*; Ooie Toshihiko; Yamachoshi Yuji; Nakahara Tomonori; Hino Mami; Akamine Rie; Kataoka Masatoshi
来源:Journal of Laser Micro Nanoengineering, 2010, 5(1): 35-38.
DOI:10.2961/jlmn.2010.01.0008

摘要

A novel micro immunoassay chip for detecting biomarkers in blood plasma has been developed. Since multiple detectors were made in a single channel, the chip was able to detect various biomarkers simultaneously. UV Laser processing of the substrate was performed to enhance immobilization of the antibody. This simple plastic chip requires only 1 micro liter per lane of blood plasma and 30 min of reaction time, one twentieth the sample volume required for the ELISA-kit and six times the through put.

  • 出版日期2010-2