Limited versus total epithelial debridement ocular surface injury: Live fluorescence imaging of hemangiogenesis and lymphangiogenesis in Prox1-GFP/Flk1: :Myr-mCherry mice

作者：Chang Jin Hong^*; Putra Ilham; Huang Yu hui; Chang Michael; Han Kyuyeon; Zhong Wei; Gao Xinbo; Wang Shuangyong; Dugas Ford Jennifer; Tara Nguyen; Hong Young Kwon; Azar Dimitri T^*

来源：Biochimica et Biophysica Acta-General Subjects, 2016, 1860(10): 2148-2156.

DOI：10.1016/j.bbagen.2016.05.027

• Cornea
• Bulbar conjunctiva
• Corneal neovascularization (NV)
• Hemangiogenesis (HA)
• Lymphangiogenesis (LA)
• In vivo imaging

Background: Immunohistochemical staining experiments have shown that both hemangiogenesis and lymphangiogenesis occur following severe corneal and conjunctival injury and that the neovascularization of the cornea often has severe visual consequences. To better understand how hemangiogenesis and lymphangiogenesis are induced by different degrees of ocular injury, we investigated patterns of injury-induced corneal neovascularization in live Prox1-GFP/Flk1::myr-mCheny mice, in which blood and lymphatic vessels can be imaged simultaneously in vivo.
Methods: The eyes of Prox1-GFP/Flk1 ::myr-mCheny mice were injured according to four models based on epithelial debridement of the: A) central cornea (a 1.5-mm-diameter circle of tissue over the corneal apex), B) total cornea, C) bulbar conjunctiva, and D) cornea + bulbar conjunctiva. Corneal blood and lymphatic vessels were imaged on days 0, 3, 7, and 10 post-injury, and the percentages of the cornea containing blood and lymphatic vessels were calculated.
Results: Neither central corneal nor bulbar conjunctival debridement resulted in significant vessel growth in the mouse cornea, whereas total corneal and corneal + bulbar conjunctival debridement did. On day 10 in the central cornea, total cornea, bulbar conjunctiva, and corneal + bulbar conjunctival epithelial debridement models, the percentage of the corneal surface that was occupied by blood vessels (hemangiogenesis) was 1.9 +/- 0.8%, 7.14 +/- 2.4%, 2.29 +/- 1%, and 15.05 +/- 2.14%, respectively, and the percentage of the corneal surface that was occupied by lymphatic vessels (lymphangiogenesis) was 2.45 +/- 1.51%, 4.85 +/- 0.95%, 2.95 +/- 1.27%, and 4.15 +/- 3.85%, respectively.
Conclusions: Substantial corneal debridement was required to induce corneal neovascularization in the mouse cornea, and the corneal epithelium may therefore be partially responsible for maintaining corneal avascularity.
General significance: Our study demonstrates that GFP/Flk1 ::myr-mCherry mice are a useful model for studying coordinated hemangiogenic and lymphangiogenic responses. Published by Elsevier B.V.

• 出版日期2016-10